Objectives: Medicinal herbs have long been used for the treatment of hepatic disorders. Rumex acetosella is one of the plants used for this purpose in the Iranian traditional medicine. This study aimed to investigate the protective effects of Rumex acetosella against the hepatotoxicity induced by carbon tetrachloride (CCl₄) in rats. Materials and Methods: In this study, 64 albino Wistar rats were divided into 8 groups. Groups I-IV were considered as control and received normal saline (I and III) and vehicle (II and IV). Groups V-VIII received 250, 500, 750 and 1500 mg/kg of Rumex acetosella, respectively through gavage for 14 days. On day 15, groups III-VIII received one ml/kg of CCl₄ 1: 1 in olive oil via intraperitoneal injection. Animals were killed 48 hours after CCl₄ injection, and their liver and blood samples were collected to determine the levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST …