The aim of this study is to assess the cytotoxic effects of partially purified cytotoxic peptides of Naja naja oxiana venom (PPCPNnov) in human Glioblastoma cell line (U-87MG cells). PPCPNnov were prepared by gel filtration of crude venom. U-87MG cells were exposed to different concentrations of PPCPNnov (10, 13/5 and 17 µg/ml). Results showed that PPCPNnov dose-dependently increased the reactive oxygen species (ROS) generation and the percentage of apoptotic cells. Apoptosis at the concentration of 17 µg/ml was accompanied by an increase in the ratio of mRNA expression of BAX/BCL-2. It seems the production of hydrogen peroxide (H2O2) during the enzymatic reaction causing activation of caspase and/or the interaction of PPCPNnov with membrane receptors leading to the cell death process that should be further evaluated.